A triple fluorescent marker for live imaging of plant cell morphogenesis
Abstract
Live imaging of plant subcellular structures is key to deciphering the spatiotemporal bases of cellular processes, and their functional impact on growth and morphogenesis at various biological scales. Live imaging of plant cells essentially relies on expression of fluorescent markers labeling cells or subcellular structures of interest. Simultaneous multi-channel imaging of several markers is still not routine practice in plant cell biology, owing to issues linked to genetic or spectral compatibility of markers, differences in expression levels, silencing, toxicity, etc. Here we designed a three-color marker in Arabidopsis thaliana and Capsella rubella , enabling high-resolution live imaging of plant morphogenesis, including labeling of the cell membrane, the nucleus and the microtubule cytoskeleton. Detection of MT arrays involved the development of a MAP4-MBD-based microtubule marker optimized for plant cells. The three- color marker allows visualization of the three-dimensional organization and dynamics of plant microtubules within the intracellular space with unprecedented precision, in various organs including the root and shoot meristems, the leaf, anther, and gynoecium. Our results demonstrate the potential of such single-construct strategy for cell biology studies in plants.
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BibTeX
@misc{bomsel2026triple,
title = {{A triple fluorescent marker for live imaging of plant cell morphogenesis}},
author = {Zoé Bomsel and Coralie Goncalves and Aloïse Ducamp and Loris Caillat-Miousse and Bérengère Dalmais and Katia Belcram and Chie Kodera and Claire Lionnet and Camila Goldy and Samantha Moulin and Marie-Cécile Caillaud and David Bouchez and Martine Pastuglia and Magalie Uyttewaal},
howpublished = {HAL},
year = {2026},
month = aug,
doi = {10.64898/2026.08.20.745988},
url = {https://hal.inrae.fr/hal-05748714v1},
note = {CC BY-NC},
}
RIS
TY - UNPB
TI - A triple fluorescent marker for live imaging of plant cell morphogenesis
AU - Zoé Bomsel
AU - Coralie Goncalves
AU - Aloïse Ducamp
AU - Loris Caillat-Miousse
AU - Bérengère Dalmais
AU - Katia Belcram
AU - Chie Kodera
AU - Claire Lionnet
AU - Camila Goldy
AU - Samantha Moulin
AU - Marie-Cécile Caillaud
AU - David Bouchez
AU - Martine Pastuglia
AU - Magalie Uyttewaal
PY - 2026
DA - 2026/08/21
PB - HAL
DO - 10.64898/2026.08.20.745988
UR - https://hal.inrae.fr/hal-05748714v1
AB - Live imaging of plant subcellular structures is key to deciphering the spatiotemporal bases of cellular processes, and their functional impact on growth and morphogenesis at various biological scales. Live imaging of plant cells essentially relies on expression of fluorescent markers labeling cells or subcellular structures of interest. Simultaneous multi-channel imaging of several markers is still not routine practice in plant cell biology, owing to issues linked to genetic or spectral compatibility of markers, differences in expression levels, silencing, toxicity, etc. Here we designed a three-color marker in Arabidopsis thaliana and Capsella rubella , enabling high-resolution live imaging of plant morphogenesis, including labeling of the cell membrane, the nucleus and the microtubule cytoskeleton. Detection of MT arrays involved the development of a MAP4-MBD-based microtubule marker optimized for plant cells. The three- color marker allows visualization of the three-dimensional organization and dynamics of plant microtubules within the intracellular space with unprecedented precision, in various organs including the root and shoot meristems, the leaf, anther, and gynoecium. Our results demonstrate the potential of such single-construct strategy for cell biology studies in plants.
ER -