PreprintCC BY-NC-NDbioRxiv
Steric attenuation of membrane-associated dimerization-induced fluorescence for cell-free biosensing
Abstract
We present a membrane-associated, cell-free biosensing reporter based on rapalog-induced split sfCherry reconstitution. Rapalog increased fluorescence in solution, lipid vesicles, and supported lipid bilayers. Antibodies binding to epitope tags near the dimerization interface reduced the fluorescence signal, consistent with steric interference with reporter assembly.
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BibTeX
@misc{parvizian2026steric,
title = {{Steric attenuation of membrane-associated dimerization-induced fluorescence for cell-free biosensing}},
author = {Parsa Parvizian and Hossein Moghimianavval and Allen P Liu},
howpublished = {bioRxiv},
year = {2026},
month = oct,
doi = {10.64898/2026.10.19.760250},
url = {https://www.biorxiv.org/content/10.64898/2026.10.19.760250v1},
note = {CC BY-NC-ND},
}
RIS
TY - UNPB
TI - Steric attenuation of membrane-associated dimerization-induced fluorescence for cell-free biosensing
AU - Parsa Parvizian
AU - Hossein Moghimianavval
AU - Allen P Liu
PY - 2026
DA - 2026/10/19
PB - bioRxiv
DO - 10.64898/2026.10.19.760250
UR - https://www.biorxiv.org/content/10.64898/2026.10.19.760250v1
AB - We present a membrane-associated, cell-free biosensing reporter based on rapalog-induced split sfCherry reconstitution. Rapalog increased fluorescence in solution, lipid vesicles, and supported lipid bilayers. Antibodies binding to epitope tags near the dimerization interface reduced the fluorescence signal, consistent with steric interference with reporter assembly.
SN - 2692-8205
ER -