Valorization of orange peel by-products via ultrasound-pretreated enzymatic extraction of soluble dietary fiber: Structural and functional characterization
- 1Faculty of Health Sciences, University Institution Colegio Mayor de Antioquia, Medellín, Antioquia, Colombia
- 2Programa de Pós-Graduação em Engenharia Química, Universidade Federal da Bahia, Salvador, Brazil
- 3Bioinformatics, Computational Biology and Data Science Research Unit, Institute of Agrochemistry and Food Technology (IATA-CSIC), Paterna-Valencia, Spain
Abstract
Agro-industrial by-products valorization has attracted increasing attention due to their potential as sustainable sources of bioactive compounds, particularly dietary fibers. In this study, orange peel by-products were used as a raw material for the recovery of soluble dietary fiber via enzymatic extraction and ultrasound-pretreated enzymatic extraction. Ultrasound-pretreated enzymatic extraction achieved a soluble dietary fiber recovery of 26.04 ± 0.81% with approximately 90% purity. The content of phenolic compounds bound to soluble dietary fiber ranged from 3.23 ± 0.03 to 12.15 ± 0.21 mg GAE g-1. Soluble dietary fiber obtained by alkaline extraction exhibited higher water-holding capacity (9.16 ± 0.08 g g -1). Enzymatic extraction caused structural modifications and partial depolymerization, reducing fiber porosity and water affinity. Oil-holding capacity ranged from 1.08 ± 0.12 to 1.24 ± 0.05 g g-1, with no differences among extraction methods. Ultrasound-pretreated enzymatic extraction produced soluble dietary fiber with a more amorphous structure, lower crystallinity, greater surface disruption, and higher thermal stability. Moreover, it is a suitable substrate for the growth of mammalian intestinal symbionts such as Faecalibaculum rodentium and Bacteroides thetaiotaomicron, species recognized as beneficial for host health. Ultrasound pretreatment is a promising strategy for recovering high-value soluble dietary fibers from orange peel by-products, thereby enabling the production of tailored functional ingredients for food applications.
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BibTeX
@article{quinonezensuncho2026valorization,
title = {{Valorization of orange peel by-products via ultrasound-pretreated enzymatic extraction of soluble dietary fiber: Structural and functional characterization}},
author = {Jhonatan D. Quiñonez-Ensuncho and Gala Rosales and Laura Ortega-Ruiz and Alfonso Benítez-Páez and Beatriz E. Valdés-Duque and Álvaro S. Lima and J. Felipe Osorio-Tobón},
journal = {PLOS One},
year = {2026},
month = sep,
doi = {10.1371/journal.pone.0357376},
url = {https://doi.org/10.1371/journal.pone.0357376},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Valorization of orange peel by-products via ultrasound-pretreated enzymatic extraction of soluble dietary fiber: Structural and functional characterization
AU - Jhonatan D. Quiñonez-Ensuncho
AU - Gala Rosales
AU - Laura Ortega-Ruiz
AU - Alfonso Benítez-Páez
AU - Beatriz E. Valdés-Duque
AU - Álvaro S. Lima
AU - J. Felipe Osorio-Tobón
PY - 2026
DA - 2026/09/01
JO - PLOS One
DO - 10.1371/journal.pone.0357376
UR - https://doi.org/10.1371/journal.pone.0357376
AB - Agro-industrial by-products valorization has attracted increasing attention due to their potential as sustainable sources of bioactive compounds, particularly dietary fibers. In this study, orange peel by-products were used as a raw material for the recovery of soluble dietary fiber via enzymatic extraction and ultrasound-pretreated enzymatic extraction. Ultrasound-pretreated enzymatic extraction achieved a soluble dietary fiber recovery of 26.04 ± 0.81% with approximately 90% purity. The content of phenolic compounds bound to soluble dietary fiber ranged from 3.23 ± 0.03 to 12.15 ± 0.21 mg GAE g-1. Soluble dietary fiber obtained by alkaline extraction exhibited higher water-holding capacity (9.16 ± 0.08 g g -1). Enzymatic extraction caused structural modifications and partial depolymerization, reducing fiber porosity and water affinity. Oil-holding capacity ranged from 1.08 ± 0.12 to 1.24 ± 0.05 g g-1, with no differences among extraction methods. Ultrasound-pretreated enzymatic extraction produced soluble dietary fiber with a more amorphous structure, lower crystallinity, greater surface disruption, and higher thermal stability. Moreover, it is a suitable substrate for the growth of mammalian intestinal symbionts such as Faecalibaculum rodentium and Bacteroides thetaiotaomicron, species recognized as beneficial for host health. Ultrasound pretreatment is a promising strategy for recovering high-value soluble dietary fibers from orange peel by-products, thereby enabling the production of tailored functional ingredients for food applications.
SN - 1932-6203
ER -