Ultrasound-assisted deep eutectic solvent extraction of polyphenols from Cornus officinalis: Optimization, mechanisms, and bioactivity
6 affiliations
- 1Department of Pharmacy, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, PR China; Wuhan University School of Pharmaceutical Sciences, Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, China
- 2Department of Psychiatry of Wuhan Wudong Hospital, Wuhan, Hubei 430085, PR China
- 3Department of Pharmacy, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, PR China
- 4Department of Pharmacy, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, PR China; Wuhan University School of Pharmaceutical Sciences, Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, China. Electronic address: [email protected]
- 5Sinopharm Dongfeng General Hospital, Hubei University of Medicine, Shiyan, Hubei 442001, PR China; Hubei Key Laboratory of Wudang Local Chinese Medicine Research, School of Pharmaceutical Sciences, Hubei University of Medicine, Shiyan, Hubei 442000, China. Electronic address: [email protected]
- 6Department of Pharmacy, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, PR China; Health Management Center, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, PR China. Electronic address: [email protected]
Abstract
This study investigated the combined use of deep eutectic solvents (DESs) and ultrasound-assisted extraction (UAE) for extracting polyphenols from Cornus officinalis (COF). The results revealed that DESs containing choline chloride-acetamide (ChCl-Ace, 1 : 2) yielded the highest extraction efficiency. Optimal extraction conditions included an ultrasonic power of 350 W, a liquid-to-solid ratio of 24 : 1 mL/g, and 45% water content. Eight phenolic compounds in the COF extract were identified using high-performance liquid chromatography-mass spectrometry. According to the SEM observations, the surface structure of the samples was modified by UAE, as indicated by an average pore size of 770 ± 14.32 nm in ChCl-Ace-UAE, which helped release phenolic compounds. Molecular dynamics simulations showed that ChCl-Ace significantly enhanced solute-solvent interactions, particularly with gallic acid and cyanidin-3-O glucoside, leading to improved extraction efficiency. In vitro chemical and cellular assays confirmed that DES-Ace displayed superior antioxidant properties. In addition, the ChCl-Ace-UAE extract (0.5 mg/mL) demonstrated 91.25 ± 0.15% Staphylococcus aureus, 95.48 ± 0.17% Escherichia coli and 93.72 ± 0.36% Propionibacterium acnes inhibition in the antibacterial assay. In summary, the combined use of UAE and DESs offers a promising and eco-friendly approach for extracting valuable compounds from botanical sources.
Related
- Ultrasound-Assisted Enzymatic Extraction of Flavonoids from Pouteria caimito Leaves: Process Optimization, Mechanistic Investigation, and Bioactivity Evaluation
- Ultrasound-assisted extraction of flavonoids from Artemisia argyi folium using deep eutectic solvents: optimization, mechanistic characterization, bioactivity evaluation, and strawberry preservation applications
- Ultrasound-assisted cloud point extraction of phenolamide from rose residues: optimization, characterization and molecular interactions
- Ultrasound-Assisted Extraction of Bioactive Compounds from Acmella oleracea L. Leaves: Optimization by Response Surface Methodology and Comparison with Percolation
- Efficiency of Innovative Extraction Technologies in Recovering Natural Pigments, Polyphenols, and Antioxidants from Sugar Beet Leaves
BibTeX
@article{chen2026ultrasoundassisted,
title = {{Ultrasound-assisted deep eutectic solvent extraction of polyphenols from Cornus officinalis: Optimization, mechanisms, and bioactivity}},
author = {Ping Chen and Wenhao Xiao and 李瑞祥 and Benhong Zhou and 陈富超 and Ruhong Zhang},
journal = {Ultrasonics Sonochemistry},
year = {2026},
month = jun,
doi = {10.1016/j.ultsonch.2026.107909},
url = {https://doi.org/10.1016/j.ultsonch.2026.107909},
note = {CC BY},
}
RIS
TY - JOUR
TI - Ultrasound-assisted deep eutectic solvent extraction of polyphenols from Cornus officinalis: Optimization, mechanisms, and bioactivity
AU - Ping Chen
AU - Wenhao Xiao
AU - 李瑞祥
AU - Benhong Zhou
AU - 陈富超
AU - Ruhong Zhang
PY - 2026
DA - 2026/06/01
JO - Ultrasonics Sonochemistry
DO - 10.1016/j.ultsonch.2026.107909
UR - https://doi.org/10.1016/j.ultsonch.2026.107909
AB - This study investigated the combined use of deep eutectic solvents (DESs) and ultrasound-assisted extraction (UAE) for extracting polyphenols from Cornus officinalis (COF). The results revealed that DESs containing choline chloride-acetamide (ChCl-Ace, 1 : 2) yielded the highest extraction efficiency. Optimal extraction conditions included an ultrasonic power of 350 W, a liquid-to-solid ratio of 24 : 1 mL/g, and 45% water content. Eight phenolic compounds in the COF extract were identified using high-performance liquid chromatography-mass spectrometry. According to the SEM observations, the surface structure of the samples was modified by UAE, as indicated by an average pore size of 770 ± 14.32 nm in ChCl-Ace-UAE, which helped release phenolic compounds. Molecular dynamics simulations showed that ChCl-Ace significantly enhanced solute-solvent interactions, particularly with gallic acid and cyanidin-3-O glucoside, leading to improved extraction efficiency. In vitro chemical and cellular assays confirmed that DES-Ace displayed superior antioxidant properties. In addition, the ChCl-Ace-UAE extract (0.5 mg/mL) demonstrated 91.25 ± 0.15% Staphylococcus aureus, 95.48 ± 0.17% Escherichia coli and 93.72 ± 0.36% Propionibacterium acnes inhibition in the antibacterial assay. In summary, the combined use of UAE and DESs offers a promising and eco-friendly approach for extracting valuable compounds from botanical sources.
SN - 1350-4177
SN - 1873-2828
ER -