Elucidating gut microbiota-targeted carbohydrate metabolic functions by high-pressure extracted polysaccharides from
5 affiliations
- 1Interdisciplinary Graduate Program in Bioscience, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand
- 2Department of Food Science and Technology, Faculty of Agro-Industry, Kasetsart University, Bangkok, 10900, Thailand
- 3School of Food Science and Engineering, Key Laboratory of Food Nutrition and Functional Food of Hainan Province, Hainan University, Haikou, 570228, China
- 4Department of Biotechnology and Biomedicine, Technical University of Denmark, Søltofts Plads, Building 223, Kongens Lyngby, 2800, Denmark
- 5Department of Food Science and Technology, Faculty of Agro-Industry, Kasetsart University, Bangkok, 10900, Thailand; Corresponding author.
Abstract
Polysaccharides in Morchella esculenta exhibit notable prebiotic potential; however, the gut microbial metabolic responses to high-pressure extracted polysaccharides (HPPs) remain poorly understood. Cooked M. esculenta powders (CMPs) were included to represent the commonly consumed form and the intact mushroom matrix. This study investigated gut microbiota-targeted metabolic functions in response to CMPs and HPPs followed by in vitro gastrointestinal digestion and fermentation. High-pressure extraction elevated β -glucan content (42.36 ± 1.51%) and generated a carbohydrate-enriched fraction of 325 kDa. During digestion, HPPs underwent a reduction in molecular weight and changes in monosaccharide composition, resulting in enhanced digestibility (35.24 ± 1.73%). The HPPs promoted short-chain fatty acids production and selectively modulated gut microbiota, such as Bacteroides, Bifidobacterium , and Acidaminococcus . Predictive functional profiling suggested enrichment in carbohydrate metabolism pathways, particularly galactose, mannose, propanoate and butanoate metabolism. This study serves the development of M. esculenta as functional food ingredients in regulating gut microbiota composition.
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BibTeX
@article{liu2026elucidating,
title = {{Elucidating gut microbiota-targeted carbohydrate metabolic functions by high-pressure extracted polysaccharides from}},
author = {Bingshu Liu and Yuke He and Putsawee Tomtong and Ruimin Wang and Solange I. Mussatto and Suvimol Charoensiddhi and Wanwipa Vongsangnak},
journal = {Food Hydrocolloids for Health},
year = {2026},
month = oct,
doi = {10.1016/j.fhfh.2026.100312},
url = {https://doi.org/10.1016/j.fhfh.2026.100312},
note = {CC BY},
}
RIS
TY - JOUR
TI - Elucidating gut microbiota-targeted carbohydrate metabolic functions by high-pressure extracted polysaccharides from
AU - Bingshu Liu
AU - Yuke He
AU - Putsawee Tomtong
AU - Ruimin Wang
AU - Solange I. Mussatto
AU - Suvimol Charoensiddhi
AU - Wanwipa Vongsangnak
PY - 2026
DA - 2026/10/30
JO - Food Hydrocolloids for Health
DO - 10.1016/j.fhfh.2026.100312
UR - https://doi.org/10.1016/j.fhfh.2026.100312
AB - Polysaccharides in Morchella esculenta exhibit notable prebiotic potential; however, the gut microbial metabolic responses to high-pressure extracted polysaccharides (HPPs) remain poorly understood. Cooked M. esculenta powders (CMPs) were included to represent the commonly consumed form and the intact mushroom matrix. This study investigated gut microbiota-targeted metabolic functions in response to CMPs and HPPs followed by in vitro gastrointestinal digestion and fermentation. High-pressure extraction elevated β -glucan content (42.36 ± 1.51%) and generated a carbohydrate-enriched fraction of 325 kDa. During digestion, HPPs underwent a reduction in molecular weight and changes in monosaccharide composition, resulting in enhanced digestibility (35.24 ± 1.73%). The HPPs promoted short-chain fatty acids production and selectively modulated gut microbiota, such as Bacteroides, Bifidobacterium , and Acidaminococcus . Predictive functional profiling suggested enrichment in carbohydrate metabolism pathways, particularly galactose, mannose, propanoate and butanoate metabolism. This study serves the development of M. esculenta as functional food ingredients in regulating gut microbiota composition.
SN - 2667-0259
ER -