A glycosaminoglycan degrading Polysaccharide Utilization Locus in the oral microbiota species Segatella oris
Abstract
The human gut microbiota has been explored for the last two decades and studies have uncovered its major role in the degradation of complex glycans from the diet as well as the host tissues. Strikingly, until very recently, no similar investigation has been carried out on the human oral cavity microbiota, which is increasingly recognized has an important parameter in gastrointestinal health. Prevotellaceae are an important and under-investigated family of bacteria in both the gut and the oral cavity microbiota. The oral cavity is particularly rich in glycosaminoglycans which could provide an abundant carbon source for the inhabiting microorganisms, and play a significant role in host-pathogen interactions. Hence, we analyzed Polysaccharide Utilization Loci in oral microbiota species and we identified a glycosaminoglycan-degrading Polysaccharide Utilization Locus in Segatella oris. We produced and characterized the four enzymes, confirmed their ability to degrade glycosaminoglycans, and we focused our efforts on the biochemical and structural characterization of the first reported Prevotellaceae endosulfatase.
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BibTeX
@misc{cousin2026glycosaminoglycan,
title = {{A glycosaminoglycan degrading Polysaccharide Utilization Locus in the oral microbiota species Segatella oris}},
author = {Abel Cousin and Yara Awad and Rebecca-Joe Boustany and Rosa Lorizolla Cordeiro and Melanie Friedel-Arboleas and Sophie Drouillard and Melanie Loiodice and Laurine Buon and Chrystel Lopin-Bon and Rebekka Wild and William Helbert and Romain Vives and Marie Couturier},
howpublished = {HAL},
year = {2026},
month = oct,
doi = {10.64898/2026.10.07.757250},
url = {https://hal.science/hal-05793575v1},
note = {CC BY},
}
RIS
TY - UNPB
TI - A glycosaminoglycan degrading Polysaccharide Utilization Locus in the oral microbiota species Segatella oris
AU - Abel Cousin
AU - Yara Awad
AU - Rebecca-Joe Boustany
AU - Rosa Lorizolla Cordeiro
AU - Melanie Friedel-Arboleas
AU - Sophie Drouillard
AU - Melanie Loiodice
AU - Laurine Buon
AU - Chrystel Lopin-Bon
AU - Rebekka Wild
AU - William Helbert
AU - Romain Vives
AU - Marie Couturier
PY - 2026
DA - 2026/10/07
PB - HAL
DO - 10.64898/2026.10.07.757250
UR - https://hal.science/hal-05793575v1
AB - The human gut microbiota has been explored for the last two decades and studies have uncovered its major role in the degradation of complex glycans from the diet as well as the host tissues. Strikingly, until very recently, no similar investigation has been carried out on the human oral cavity microbiota, which is increasingly recognized has an important parameter in gastrointestinal health. Prevotellaceae are an important and under-investigated family of bacteria in both the gut and the oral cavity microbiota. The oral cavity is particularly rich in glycosaminoglycans which could provide an abundant carbon source for the inhabiting microorganisms, and play a significant role in host-pathogen interactions. Hence, we analyzed Polysaccharide Utilization Loci in oral microbiota species and we identified a glycosaminoglycan-degrading Polysaccharide Utilization Locus in Segatella oris. We produced and characterized the four enzymes, confirmed their ability to degrade glycosaminoglycans, and we focused our efforts on the biochemical and structural characterization of the first reported Prevotellaceae endosulfatase.
ER -