Temperature-Responsive Antheraea pernyi Silk Fibroin/Poly(N-isopropylacrylamide) Hydrogels for Controlled Protein Drug Delivery.
- 1Liaoning Provincial Key Laboratory of Functional Textile Materials, Liaodong University, Dandong 118001, China.
- 2School of Textiles and Apparel, Liaodong University, Dandong 118001, China.
Abstract
Temperature-responsive hydrogels provide hydrated matrices for regulating protein release near physiological temperature. Building on a previously developed p(ASF-AGE-NIPAAm) hydrogel platform, this study evaluated five formulations with ASF-AGE feed contents of 10.0-57.1 wt% as protein delivery carriers. The hydrogels exhibited lower critical solution temperatures (LCSTs) of 31.8-33.0 °C and reversible swelling-deswelling transitions during cyclic temperature switching between 20 and 40 °C. Increasing the ASF-AGE content decreased the equilibrium swelling ratio from 17.3 to 10.6 g/g, while the bovine serum albumin (BSA) encapsulation efficiency varied between 73.5% and 76.8% and the drug loading capacity decreased from 452.6 to 380.2 mg/g. BSA release was evaluated in phosphate-buffered saline (PBS, pH 7.4) and depended on both temperature and formulation: the apparent cumulative release at the end of the observation period ranged from 37.6% to 54.1% at 20 °C and from 38.4% to 61.2% at 37 °C. Release was higher at 37 °C, coinciding with thermally induced network contraction, and ASF-rich formulations generally released less BSA. These Results define a composition-property-release relationship for p(ASF-AGE-NIPAAm) hydrogels and show that the ASF content can be adjusted to balance protein loading against temperature-responsive release.
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BibTeX
@article{wang2026temperatureresponsive,
title = {{Temperature-Responsive Antheraea pernyi Silk Fibroin/Poly(N-isopropylacrylamide) Hydrogels for Controlled Protein Drug Delivery.}},
author = {Boxiang Wang and Hangdan Xu and Hong Huang and Jinyang Xie and Huihui Liu},
journal = {Gels (Basel, Switzerland)},
year = {2026},
month = oct,
doi = {10.3390/gels12100888},
url = {https://europepmc.org/article/MED/42929921},
note = {CC BY},
}
RIS
TY - JOUR
TI - Temperature-Responsive Antheraea pernyi Silk Fibroin/Poly(N-isopropylacrylamide) Hydrogels for Controlled Protein Drug Delivery.
AU - Boxiang Wang
AU - Hangdan Xu
AU - Hong Huang
AU - Jinyang Xie
AU - Huihui Liu
PY - 2026
DA - 2026/10/01
JO - Gels (Basel, Switzerland)
DO - 10.3390/gels12100888
UR - https://europepmc.org/article/MED/42929921
AB - Temperature-responsive hydrogels provide hydrated matrices for regulating protein release near physiological temperature. Building on a previously developed p(ASF-AGE-NIPAAm) hydrogel platform, this study evaluated five formulations with ASF-AGE feed contents of 10.0-57.1 wt% as protein delivery carriers. The hydrogels exhibited lower critical solution temperatures (LCSTs) of 31.8-33.0 °C and reversible swelling-deswelling transitions during cyclic temperature switching between 20 and 40 °C. Increasing the ASF-AGE content decreased the equilibrium swelling ratio from 17.3 to 10.6 g/g, while the bovine serum albumin (BSA) encapsulation efficiency varied between 73.5% and 76.8% and the drug loading capacity decreased from 452.6 to 380.2 mg/g. BSA release was evaluated in phosphate-buffered saline (PBS, pH 7.4) and depended on both temperature and formulation: the apparent cumulative release at the end of the observation period ranged from 37.6% to 54.1% at 20 °C and from 38.4% to 61.2% at 37 °C. Release was higher at 37 °C, coinciding with thermally induced network contraction, and ASF-rich formulations generally released less BSA. These Results define a composition-property-release relationship for p(ASF-AGE-NIPAAm) hydrogels and show that the ASF content can be adjusted to balance protein loading against temperature-responsive release.
SN - 2310-2861
ER -