Intravital 3-photon microscopy of leukocyte dynamics in the splenic white pulp
- 1Department of Microbiology and Immunology, The University of Melbourne, The Peter Doherty Institute for Infection and Immunity, Melbourne, VIC, Australia
Abstract
Intravital imaging has been instrumental in uncovering the complex behaviors of immune cells during immune responses against viruses and tumors. The spleen is a major hub for systemic immune responses and immune memory. However, imaging of the spleen remains technically challenging, limiting our understanding of the dynamics of cellular interactions within specialized spleen niches, including the white pulp. Here, we present a method to stabilize the spleen for intravital imaging and to capture cell dynamics of the white pulp using 3-photon microscopy. We detail how to process images, quantify cell migration, and integrate behavior with structural information, revealing stromal guidance of T cell migration within deep T cell zones. This method of intravital 3-photon imaging of splenic white pulp supports in-depth investigation of cell dynamics and interactions in health and disease.
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BibTeX
@article{schienstock2026intravital,
title = {{Intravital 3-photon microscopy of leukocyte dynamics in the splenic white pulp}},
author = {Dominik Schienstock and Sapna Devi and Scott N. Mueller},
journal = {Cell Reports Methods},
year = {2026},
month = sep,
doi = {10.1016/j.crmeth.2026.101609},
url = {https://doi.org/10.1016/j.crmeth.2026.101609},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Intravital 3-photon microscopy of leukocyte dynamics in the splenic white pulp
AU - Dominik Schienstock
AU - Sapna Devi
AU - Scott N. Mueller
PY - 2026
DA - 2026/09/25
JO - Cell Reports Methods
DO - 10.1016/j.crmeth.2026.101609
UR - https://doi.org/10.1016/j.crmeth.2026.101609
AB - Intravital imaging has been instrumental in uncovering the complex behaviors of immune cells during immune responses against viruses and tumors. The spleen is a major hub for systemic immune responses and immune memory. However, imaging of the spleen remains technically challenging, limiting our understanding of the dynamics of cellular interactions within specialized spleen niches, including the white pulp. Here, we present a method to stabilize the spleen for intravital imaging and to capture cell dynamics of the white pulp using 3-photon microscopy. We detail how to process images, quantify cell migration, and integrate behavior with structural information, revealing stromal guidance of T cell migration within deep T cell zones. This method of intravital 3-photon imaging of splenic white pulp supports in-depth investigation of cell dynamics and interactions in health and disease.
SN - 2667-2375
ER -