Synergistic ultrasound- and vortex-assisted extraction of lobetyolin from Codonopsis tangshen using a supramolecular ternary deep eutectic solvent: yield optimization and antioxidant activity evaluation
5 affiliations
- 1College of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, China
- 2Chongqing Academy of Chinese Materica Medica, Chongqing, China
- 3Agricultural Development Service Center of Kaizhou District, Chongqing, China
- 4Shandong Baiweitang Traditional Chinese Medicine Decoction Pieces Co., Ltd., China
- 5Agricultural Technology Extension Center of Pingyi County, Linyi City, Shandong Province, China
Abstract
Herein, a supramolecular ternary deep eutectic solvent coupled with in-situ ultrasound-assisted extraction (STDES-ISUSE) was developed to extract lobetyolin (LBT) in Codonopsis tangshen. The hydroxypropyl-β-cyclodextrin: lactic acid: glycerol (HP-β-CD: LA: Gly) system stood out to extract LBT among the twenty formulations tested. The optimal extraction conditions—water content of 46% (v/v), liquid/solid ratio of 29 mL/g, ultrasound time of 34 min, ultrasound power of 240 W, and vortex time of 10 min—were established via a hybrid response surface methodology-artificial neural network-genetic algorithm (RSM-ANN-GA) paradigm. Under these conditions, the LBT yield reached 0.613 ± 0.011 mg/g, significantly exceeding that of conventional methods. Twenty compounds were identified via UHPLC-Q-TOF-MS, encompassing two phenolic acids, two polyacetylenic glycosides, five fatty acids, two glycosides, one iridoids, and one alkaloids, while a tailored HPLC method enabled precise LBT quantification. Mechanistic investigations, combining FT-IR corroboration of STDES fabrication, SEM visualization, and MD simulations, collectively revealed that the STDES-ISUSE approach potentiates extraction through synergistic cell-wall disintegration and enhanced LBT solubilization. In vitro antioxidant testing further substantiated the bioactivity of the extracts. In summary, this work delivers a green, high-efficiency extraction protocol for bioactive targets from C. tangshen, offering a practical route toward sustainable phytochemical recovery and the broader implementation of green extraction practices in natural product research.
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BibTeX
@article{liu2026synergistic,
title = {{Synergistic ultrasound- and vortex-assisted extraction of lobetyolin from Codonopsis tangshen using a supramolecular ternary deep eutectic solvent: yield optimization and antioxidant activity evaluation}},
author = {Cheng Liu and Yiquan Zhou and Jin Guo and Yijia Chen and Xia Zhao and Zhiqiang Chen and Xin Zhao and Xiaochuan Zou and Fangyuan Gong and Ailing Lei and Shuxin He and Xin Ma and Xinyu Liu and Jie Lei},
journal = {Ultrasonics Sonochemistry},
year = {2026},
month = oct,
doi = {10.1016/j.ultsonch.2026.108126},
url = {https://doi.org/10.1016/j.ultsonch.2026.108126},
note = {CC BY-NC-ND 4.0},
}
RIS
TY - JOUR
TI - Synergistic ultrasound- and vortex-assisted extraction of lobetyolin from Codonopsis tangshen using a supramolecular ternary deep eutectic solvent: yield optimization and antioxidant activity evaluation
AU - Cheng Liu
AU - Yiquan Zhou
AU - Jin Guo
AU - Yijia Chen
AU - Xia Zhao
AU - Zhiqiang Chen
AU - Xin Zhao
AU - Xiaochuan Zou
AU - Fangyuan Gong
AU - Ailing Lei
AU - Shuxin He
AU - Xin Ma
AU - Xinyu Liu
AU - Jie Lei
PY - 2026
DA - 2026/10/21
JO - Ultrasonics Sonochemistry
DO - 10.1016/j.ultsonch.2026.108126
UR - https://doi.org/10.1016/j.ultsonch.2026.108126
AB - Herein, a supramolecular ternary deep eutectic solvent coupled with in-situ ultrasound-assisted extraction (STDES-ISUSE) was developed to extract lobetyolin (LBT) in Codonopsis tangshen. The hydroxypropyl-β-cyclodextrin: lactic acid: glycerol (HP-β-CD: LA: Gly) system stood out to extract LBT among the twenty formulations tested. The optimal extraction conditions—water content of 46% (v/v), liquid/solid ratio of 29 mL/g, ultrasound time of 34 min, ultrasound power of 240 W, and vortex time of 10 min—were established via a hybrid response surface methodology-artificial neural network-genetic algorithm (RSM-ANN-GA) paradigm. Under these conditions, the LBT yield reached 0.613 ± 0.011 mg/g, significantly exceeding that of conventional methods. Twenty compounds were identified via UHPLC-Q-TOF-MS, encompassing two phenolic acids, two polyacetylenic glycosides, five fatty acids, two glycosides, one iridoids, and one alkaloids, while a tailored HPLC method enabled precise LBT quantification. Mechanistic investigations, combining FT-IR corroboration of STDES fabrication, SEM visualization, and MD simulations, collectively revealed that the STDES-ISUSE approach potentiates extraction through synergistic cell-wall disintegration and enhanced LBT solubilization. In vitro antioxidant testing further substantiated the bioactivity of the extracts. In summary, this work delivers a green, high-efficiency extraction protocol for bioactive targets from C. tangshen, offering a practical route toward sustainable phytochemical recovery and the broader implementation of green extraction practices in natural product research.
SN - 1350-4177
SN - 1873-2828
ER -