Super-resolution mapping of nuclear stress signaling by probiotic-derived extracellular vesicles in UV-induced skin photodamage
6 affiliations
- 1Department of Chemistry, Hanyang University, Seoul 04763, Republic of Korea.
- 2Center for Bio-imaging & Translational Research, Korea Basic Science Institute, Cheongju 28119, Republic of Korea.
- 3R&D Institute, LG Household & Health Care (LG H&H), Seoul 07795, Republic of Korea.
- 4Research Institute for Convergence of Basic Science, Hanyang University, Seoul 04763, Republic of Korea.
- 5Institute of Nano Science and Technology, Hanyang University, Seoul 04763, Republic of Korea.
- 6Research Institute for Natural Sciences, Hanyang University, Seoul 04763, Republic of Korea.
Abstract
Ultraviolet (UV)-induced photodamage drives oxidative stress, DNA damage, and chronic inflammation, leading to progressive cellular ultrastructure and skin function degeneration. Despite the potential of probiotics as anti-photodamage agents, their nanoscale action mechanism remains unexplored. Here, we utilize super-resolution fluorescence microscopy to investigate the cellular and molecular basis of Lactobacillus plantarum-derived extracellular vesicles (L. plantarum EVs) in mitigating photodamage. We showed that UV irradiation triggered extensive organellar remodeling along with elevated reactive oxygen species production, DNA damage signaling, and inflammatory activation. Remarkably, pre- and posttreatment with L. plantarum EVs preserved organelle integrity and restored native nanoscale architecture. Additionally, EV treatment delayed UV-induced PARP-1 activation and nuclear condensate clustering, while suppressing NF-κB translocation and downstream inflammatory cytokine expression. These findings establish probiotic-derived EVs as modulators of stress-responsive signaling and structural homeostasis during UV-induced photodamage. By providing a super-resolution mechanistic framework for understanding probiotic EV-mediated modulation of cellular photodamage responses, this study provides a basis for future investigation of EV-based strategies for skin protection.
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BibTeX
@article{go2026superresolution,
title = {{Super-resolution mapping of nuclear stress signaling by probiotic-derived extracellular vesicles in UV-induced skin photodamage}},
author = {Ga-eun Go and Seungju Han and Minjae Kang and Sangoh Choi and Soo-eun Lee and Uidon Jeong and Sang-Hee Lee and Ki-Nam Gu and Hanji Kim and Bo Kyoung Hwang and Yunkwan Kim and Euitaek Jeong and Nae-Gyu Kang and Doory Kim},
journal = {Science Advances},
year = {2026},
month = oct,
doi = {10.1126/sciadv.aek5197},
url = {https://www.science.org/doi/abs/10.1126/sciadv.aek5197},
note = {CC BY},
}
RIS
TY - JOUR
TI - Super-resolution mapping of nuclear stress signaling by probiotic-derived extracellular vesicles in UV-induced skin photodamage
AU - Ga-eun Go
AU - Seungju Han
AU - Minjae Kang
AU - Sangoh Choi
AU - Soo-eun Lee
AU - Uidon Jeong
AU - Sang-Hee Lee
AU - Ki-Nam Gu
AU - Hanji Kim
AU - Bo Kyoung Hwang
AU - Yunkwan Kim
AU - Euitaek Jeong
AU - Nae-Gyu Kang
AU - Doory Kim
PY - 2026
DA - 2026/10/30
JO - Science Advances
DO - 10.1126/sciadv.aek5197
UR - https://www.science.org/doi/abs/10.1126/sciadv.aek5197
AB - Ultraviolet (UV)-induced photodamage drives oxidative stress, DNA damage, and chronic inflammation, leading to progressive cellular ultrastructure and skin function degeneration. Despite the potential of probiotics as anti-photodamage agents, their nanoscale action mechanism remains unexplored. Here, we utilize super-resolution fluorescence microscopy to investigate the cellular and molecular basis of Lactobacillus plantarum-derived extracellular vesicles (L. plantarum EVs) in mitigating photodamage. We showed that UV irradiation triggered extensive organellar remodeling along with elevated reactive oxygen species production, DNA damage signaling, and inflammatory activation. Remarkably, pre- and posttreatment with L. plantarum EVs preserved organelle integrity and restored native nanoscale architecture. Additionally, EV treatment delayed UV-induced PARP-1 activation and nuclear condensate clustering, while suppressing NF-κB translocation and downstream inflammatory cytokine expression. These findings establish probiotic-derived EVs as modulators of stress-responsive signaling and structural homeostasis during UV-induced photodamage. By providing a super-resolution mechanistic framework for understanding probiotic EV-mediated modulation of cellular photodamage responses, this study provides a basis for future investigation of EV-based strategies for skin protection.
SN - 2375-2548
ER -