Response Surface Optimization of Ultrasound-Assisted Deep Eutectic Solvent Extraction of Bioactive Polysaccharides From Sword Bean (Canavalia gladiata (Jacq.) DC.) Pods.
- 1School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, China.
- 2School of Medicine, Foshan University, Foshan, China.
- 3Department of Neurology, Shenzhen Bao'an District Songgang People's Hospital, Shenzhen, China.
Abstract
Safe and functional natural active ingredients are a research hotspot in early intervention studies of chronic diseases. This study focuses on a polysaccharide (CGPs‑1) from sword bean (Canavalia gladiata (Jacq.) DC.) pods. Its optimal ultrasound-assisted extraction conditions were achieved with choline chloride/oxalic acid DES (2:1), 34% water, 21 mL/g, 65°C, 29 min at 280 W; the actual yield (33.24%) was consistent with the predicted value (33.96%). CGPs‑1 was identified as a pyran‑type polysaccharide, comprising rhamnose (Rha), glucuronic acid (GlcA), and galacturonic acid (GalA) in a molar ratio of 1.08:1:1.78. CGPs‑1 had IC₅₀ values of 0.157 mg/mL (α‑glucosidase), 0.083 mg/mL (α‑amylase), 31.18 mg/mL (DPPH radicals), and 2.264 mg/mL (ABTS radicals), inhibited LPS‑triggered inflammatory response and LPS‑mediated neurotoxicity in BV2 cells, and also suppressed MPTP-induced inflammatory response in zebrafish larvae. Results indicate that CGPs‑1 exhibits antioxidant, hypoglycemic, anti‑neuroinflammatory and neuroprotective potentials, providing preliminary experimental support for further research and the development of functional‑food‑related products derived from sword bean pods.
Related
- Ultrasound-assisted deep eutectic solvent extraction of Moringa oleifera leaf polysaccharides: process optimization, purification, physicochemical characterization, and bioactivities.
- Ultrasound-Assisted Deep Eutectic Solvent Extraction of Polysaccharides from Baeckea frutescens L.: Hybrid RSM and GA–BP Optimization, Structural Characterization, and Anti-Complementary Activity
- Tailoring Garlic Bioactive Profiles Through Green Extraction: Comparative Optimization of Microwave- and Ultrasound-Assisted Technologies
- Ultrasound-assisted deep eutectic solvent extraction of polysaccharides from Melastoma dodecandrum: RSM optimization, ANN prediction, kinetics, structural characterization and cellular antioxidant activity
- Ultrasound-Assisted Extraction of Bioactive Compounds from Acmella oleracea L. Leaves: Optimization by Response Surface Methodology and Comparison with Percolation
BibTeX
@article{xing2026response,
title = {{Response Surface Optimization of Ultrasound-Assisted Deep Eutectic Solvent Extraction of Bioactive Polysaccharides From Sword Bean (Canavalia gladiata (Jacq.) DC.) Pods.}},
author = {Jieyu Xing and Linjing Zhang and Zhaoxuan Liang and Zheng Liu and Haiyun Chen and Xian Fu},
journal = {Chemistry \& biodiversity},
year = {2026},
month = oct,
doi = {10.1002/cbdv.71766},
url = {https://europepmc.org/article/MED/42904766},
note = {CC BY},
}
RIS
TY - JOUR
TI - Response Surface Optimization of Ultrasound-Assisted Deep Eutectic Solvent Extraction of Bioactive Polysaccharides From Sword Bean (Canavalia gladiata (Jacq.) DC.) Pods.
AU - Jieyu Xing
AU - Linjing Zhang
AU - Zhaoxuan Liang
AU - Zheng Liu
AU - Haiyun Chen
AU - Xian Fu
PY - 2026
DA - 2026/10/01
JO - Chemistry & biodiversity
DO - 10.1002/cbdv.71766
UR - https://europepmc.org/article/MED/42904766
AB - Safe and functional natural active ingredients are a research hotspot in early intervention studies of chronic diseases. This study focuses on a polysaccharide (CGPs‑1) from sword bean (Canavalia gladiata (Jacq.) DC.) pods. Its optimal ultrasound-assisted extraction conditions were achieved with choline chloride/oxalic acid DES (2:1), 34% water, 21 mL/g, 65°C, 29 min at 280 W; the actual yield (33.24%) was consistent with the predicted value (33.96%). CGPs‑1 was identified as a pyran‑type polysaccharide, comprising rhamnose (Rha), glucuronic acid (GlcA), and galacturonic acid (GalA) in a molar ratio of 1.08:1:1.78. CGPs‑1 had IC₅₀ values of 0.157 mg/mL (α‑glucosidase), 0.083 mg/mL (α‑amylase), 31.18 mg/mL (DPPH radicals), and 2.264 mg/mL (ABTS radicals), inhibited LPS‑triggered inflammatory response and LPS‑mediated neurotoxicity in BV2 cells, and also suppressed MPTP-induced inflammatory response in zebrafish larvae. Results indicate that CGPs‑1 exhibits antioxidant, hypoglycemic, anti‑neuroinflammatory and neuroprotective potentials, providing preliminary experimental support for further research and the development of functional‑food‑related products derived from sword bean pods.
SN - 1612-1872
SN - 1612-1880
ER -